FABP4 Polyclonal antibody

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  WB analysis of MC38 using 31129-1-AP

  WB result of FABP4 antibody (31129-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and FABP4 knockout MC38 cells.

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  WB analysis using 31129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31129-1-AP (FABP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of 3T3-L1 using 31129-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using FABP4 antibody (31129-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

FABP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31129-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, AFABP, ALBP, Fatty acid binding protein 4

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, MC38 cells, rat heart tissue, rat skeletal muscle tissue
• Positive IF/ICC detected in: 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31129-1-AP targets FABP4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0880

  Product name: Recombinant Human FABP4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 2-132 aa of NM_001442.3

  Sequence: CDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA

• Full Name: fatty acid binding protein 4, adipocyte
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: NM_001442.3
• Gene Symbol: FABP4
• Gene ID (NCBI): 2167
• RRID: AB_3669865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15090
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.