Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14928-1-AP targets FAH in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6704 Product name: Recombinant human FAH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 70-419 aa of BC002527 Sequence: FMGLGQAAWKEARVFLQNLLSVSQARLRDDTELRKCAFISQASATMHLPATIGDYTDFYSSRQHATNVGIMFRDKENALMPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGNRLGEPIPISKAHEHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQDPRPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGDGYRIGFGQCAGKVLPALLPS Predict reactive species |
| Full Name | fumarylacetoacetate hydrolase (fumarylacetoacetase) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC002527 |
| Gene Symbol | FAH |
| Gene ID (NCBI) | 2184 |
| RRID | AB_2246738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16930 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAH antibody 14928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FAH Polyclonal antibody (Cat# 14928-1-AP) has 12 citations published in journals including Cell Metabolism, Nature Communications, Cell Reports, International Journal of Oncology, Life Sciences, American Journal of Pathology, International Journal of Cancer, Oncology Reports, PLoS One, Cartilage, Head & Neck, and Science Advances. Research fields span cancer biology (ovarian, hepatocellular, gastric, colorectal, thyroid, bladder, and laryngeal cancers), tyrosine metabolism, osteoarthritis, and calcium homeostasis regulation.
| Species | Application | Title |
|---|---|---|
Cell Metab Tyrosine catabolism enhances genotoxic chemotherapy by suppressing translesion DNA synthesis in epithelial ovarian cancer | ||
Nat Commun Targeting tRNA-dependent tyrosine usage unveils a metabolic vulnerability in hepatocellular carcinoma. | ||
Cell Rep HPD is a copper-binding protein that interacts with DLAT to promote colorectal cancer cuproptosis under copper stress | ||
