FAH Monoclonal antibody, PBS Only

• 

  WB analysis using 67986-1-Ig (same clone as 67986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67986-1-Ig (same clone as 67986-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67986-1-Ig (same clone as 67986-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 67986-1-Ig (same clone as 67986-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

FAH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67986-1-PBS

Clone No.2F8E11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2F8E11, Beta-diketonase, EC:3.7.1.2, FAA, Fumarylacetoacetate hydrolase

---

Product Information

67986-1-PBS targets FAH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6704

  Product name: Recombinant human FAH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 70-419 aa of BC002527

  Sequence: FMGLGQAAWKEARVFLQNLLSVSQARLRDDTELRKCAFISQASATMHLPATIGDYTDFYSSRQHATNVGIMFRDKENALMPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGNRLGEPIPISKAHEHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQDPRPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGDGYRIGFGQCAGKVLPALLPS

• Full Name: fumarylacetoacetate hydrolase (fumarylacetoacetase)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC002527
• Gene Symbol: FAH
• Gene ID (NCBI): 2184
• RRID: AB_2918735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P16930
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).