FAIM Polyclonal antibody

FAIM Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13005-1-AP

Synonyms

FAIM, FAIM1

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Validation Data Gallery

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  WB analysis of HEK-293 using 13005-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13005-1-AP (FAIM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13005-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13005-1-AP (FAIM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13005-1-AP

  IP result of anti-FAIM (IP:13005-1-AP, 4ug; Detection:13005-1-AP 1:500) with HEK-293 cells lysate 1680 ug.

• 

  IHC staining of human gliomas using 13005-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13005-1-AP (FAIM antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13005-1-AP targets FAIM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAIM fusion protein Ag3685
• Full Name: Fas apoptotic inhibitory molecule
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC012478
• Gene Symbol: FAIM
• Gene ID (NCBI): 55179
• RRID: AB_2100935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	Autophagy; FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.; Authors - Tianyu Han