FAM101A Polyclonal antibody

FAM101A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21113-1-AP

Synonyms

FAM101A, Protein FAM101A

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 21113-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21113-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21113-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 21113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 21113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 21113-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21113-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HepG2 cells, human skeletal muscle tissue, mouse spleen tissue
• Positive IHC detected in: human stomach tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21113-1-AP is FAM101A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM101A fusion protein Ag15314
• Full Name: family with sequence similarity 101, member A
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC141805
• Gene Symbol: FAM101A
• Gene ID (NCBI): 144347
• RRID: AB_10733877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.