FAM103A1 Polyclonal antibody

FAM103A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19422-1-AP

Synonyms

RNA guanine-7 methyltransferase activating subunit, RAMMET, RAMAC, RAM, HsT19360

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Validation Data Gallery

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  WB analysis of A549 using 19422-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19422-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 19422-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 19422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19422-1-AP (FAM103A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: mouse lung tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19422-1-AP targets FAM103A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM103A1 fusion protein Ag13749
• Full Name: family with sequence similarity 103, member A1
• Calculated Molecular Weight: 118 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC003627
• Gene Symbol: FAM103A1
• Gene ID (NCBI): 83640
• RRID: AB_2878578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM103A1, also named as RNMT-activating mini protein, is a 118 amino acid protein, which belongs to the RAM family. FAM103A1 as a component of mRNA cap binding complex localizes in the nucleus. FAM103A1 is required for efficient mRNA cap methylation and regulates RNMT expression by a post-transcriptional stabilizing mechanism.

Publications

Species	Application	Title
human	WB	J Mol Biol; Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.; Authors - Michael J Osborne