FAM105A Polyclonal antibody

FAM105A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25668-1-AP

Synonyms

FAM105A, NET20, Protein FAM105A

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Validation Data Gallery

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  WB analysis of COLO 320 using 25668-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25668-1-AP (FAM105A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 25668-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25668-1-AP (FAM105A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 25668-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25668-1-AP (FAM105A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 25668-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25668-1-AP (FAM105A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25668-1-AP is FAM105A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM105A fusion protein Ag22243
• Full Name: family with sequence similarity 105, member A
• Calculated Molecular Weight: 356 aa, 42 kDa
• Observed Molecular Weight: ~35 kDa
• GenBank Accession Number: BC011524
• Gene Symbol: FAM105A
• Gene ID (NCBI): 54491
• RRID: AB_2880185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.