Tested Applications
| Positive WB detected in | K-562 cells, L02 cells, THP-1 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21079-1-AP targets FAM119A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15282 Product name: Recombinant human FAM119A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 92-218 aa of BC033720 Sequence: ITDRKVALEFLKSNVQANLPPHIQTKTVVKELTWGQNLGSFSPGEFDLILGADIIYLEETFTDLLQTLEHLCSNHSVILLACRIRYERDNNFLAMLERQFIVRKVHYDPEKDVHIYEAQKRNQKEDL Predict reactive species |
| Full Name | family with sequence similarity 119, member A |
| Calculated Molecular Weight | 218 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC033720 |
| Gene Symbol | FAM119A |
| Gene ID (NCBI) | 151194 |
| RRID | AB_10755289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM119A antibody 21079-1-AP | Download protocol |
| IHC protocol for FAM119A antibody 21079-1-AP | Download protocol |
| WB protocol for FAM119A antibody 21079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FAM119A Polyclonal antibody (Cat# 21079-1-AP) has 4 citations. These appear in NPJ Precision Oncology, Journal of Biological Chemistry, Journal of Proteomics, and Oncogene. The associated research fields include hepatocellular carcinoma progression and 5-fluorouracil resistance, lysine trimethylation and endoplasmic reticulum stress, comparative proteomics of protein methylation, and methylation-driven lymphatic metastasis in gastric cancer. Applications cited span WB, IP, IHC, IF, and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
NPJ Precis Oncol METTL21A promotes hepatocellular carcinoma progression via methylating and stabilizing BAG3
| ||
J Biol Chem Lysine trimethylation regulates 78 kDa glucose-regulated protein proteostasis during endoplasmic reticulum stress. | ||
J Proteomics Comparative proteomic analysis of protein methylation provides insight into the resistance of hepatocellular carcinoma to 5-fluorouracil. | ||
Oncogene Methylation-driven SOX18 nuclear translocation by IGF2BP1-stabilized METTL21A promotes lymphatic metastasis in gastric cancer. |
