Tested Applications
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21529-1-AP targets FAM120A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16149 Product name: Recombinant human FAM120A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 285-538 aa of BC111736 Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR Predict reactive species |
| Full Name | family with sequence similarity 120A |
| Calculated Molecular Weight | 1118 aa, 122 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | BC111736 |
| Gene Symbol | FAM120A |
| Gene ID (NCBI) | 23196 |
| RRID | AB_10860261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZB2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM120A (also named OSSA/C9ORF10) is mapped to chromosome 9q22.31, which was first reported to be a component of Purα-containing mRNP complexes. FAM120A as a transcription co-activator that couples transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1-serine/arginine-rich protein kinase 2 (SRPK2) signaling. FAM120A is abundantly expressed in the cytoplasm of the GC cells and elevated FAM120A is observed in the scirrhous-type GC tissues compared with normal gastric mucosa. (PMID: 38565940, PMID: 37595559)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM120A antibody 21529-1-AP | Download protocol |
| IHC protocol for FAM120A antibody 21529-1-AP | Download protocol |
| WB protocol for FAM120A antibody 21529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FAM120A Polyclonal antibody (Cat# 21529-1-AP) has 2 citations. One appears in Molecular Neurobiology (2024), studying IGF2BP-mediated translation in puberty onset delay (rat, WB). The other is in Journal of Experimental & Clinical Cancer Research (2026), characterizing FAM120A as a novel effector in the progranulin/EphA2 oncogenic axis in bladder cancer. The associated research fields span neurobiology and oncology.
| Species | Application | Title |
|---|---|---|
Mol Neurobiol Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay | ||
J Exp Clin Cancer Res Functional characterization of FAM120A as a novel effector in the progranulin/EphA2 oncogenic axis in bladder cancer. |
Reviews
What customers say
The single review for this product reports a very clear and clean band with no unspecific bands, observed in 293A cell culture. The reviewer gave it a 5-star rating. Overall, the feedback highlights strong specificity and clean performance in cell culture applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Victoria (Verified Customer) (11-06-2025) | Band was very clear and clean. No unspecific bands
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