FAM120A Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21529-1-AP

Synonyms

C9orf10, DNAPTP1, DNAPTP5, FAM120A, KIAA0183, OSSA, Protein FAM120A

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Validation Data Gallery

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  WB analysis of siRNA cells using 21529-1-AP

  WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A.

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  WB analysis of HEK-293 using 21529-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21529-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 21529-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 21529-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21529-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21529-1-AP (FAM120A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, siRNA cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21529-1-AP is FAM120A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM120A fusion protein Ag16149
• Full Name: family with sequence similarity 120A
• Calculated Molecular Weight: 1118 aa, 122 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC111736
• Gene Symbol: FAM120A
• Gene ID (NCBI): 23196
• RRID: AB_10860261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay; Authors - Yifen Shen