FAM127 Polyclonal antibody

FAM127 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 20282-1-AP

Synonyms

CXX1, CXX1b, CXX1C, FAM127, FAM127 A/B/C, MAR8, MAR8A, MAR8B, MAR8C, RTL8, RTL8 A/B/C, RTL8A, RTL8B, RTL8C

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Validation Data Gallery

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  WB analysis of PC-3 using 20282-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 20282-1-AP

  IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug.

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  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20282-1-AP

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human colon tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

20282-1-AP targets FAM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM127 fusion protein Ag13930
• Full Name: family with sequence similarity 127, member B
• Calculated Molecular Weight: 113 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC000393
• Gene Symbol: FAM127B
• Gene ID (NCBI): 26071
• RRID: AB_10694677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

Publications

Species	Application	Title
human,mouse	WB	Cell Mol Life Sci; RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control.; Authors - Harihar Milaganur Mohan
	IP	J Biol Chem; Global proteomics of Ubqln2-based murine models of ALS.; Authors - Alexandra M Whiteley
human		bioRxiv; Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties; Authors - Harihar M Mohan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Harihar (Verified Customer) (01-28-2024)

The antibody works well for Western blots and we have validated it in knockout cell lines. There is however a lot of high molecular weight background bands. It is not suitable for IF.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 (WB)
• Cell Tissue Type: HEK293, HeLa, Kolf 2 IPSCs

FAM127 Antibody Western Blot validation (1:500 (WB) dilution) in HEK293, HeLa, Kolf 2 IPSCs (Cat no:20282-1-AP)