FAM131B Polyclonal antibody

FAM131B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26498-1-AP

Synonyms

Protein FAM131B, KIAA0773

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Validation Data Gallery

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  WB analysis of PC-3 using 26498-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 26498-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 26498-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 26498-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 26498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using FAM131B antibody (26498-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: PC-3 cells, SH-SY5Y cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26498-1-AP targets FAM131B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM131B fusion protein Ag24143
• Full Name: family with sequence similarity 131, member B
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC045611
• Gene Symbol: FAM131B
• Gene ID (NCBI): 9715
• RRID: AB_2880534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM131B, also named as KIAA0773, has some isoforms with MW 35-40 kDa.