FAM134A Polyclonal antibody, PBS Only

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• FAM134A Polyclonal antibody, PBS Only (24650-1-PBS)

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  WB analysis using 24650-1-AP (same clone as 24650-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24650-1-AP (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24650-1-AP (same clone as 24650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24650-1-AP (same clone as 24650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 24650-1-AP (same clone as 24650-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FAM134A antibody (24650-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24650-1-PBS in a different storage buffer formulation.

FAM134A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24650-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

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Product Information

24650-1-PBS targets FAM134A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20025

  Product name: Recombinant human FAM134A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 244-543 aa of BC002420

  Sequence: DYSMKAEANALHHKHDKRKRQGKNAPPGGDEPLAETESESEAELAGFSPVVDVKKTALALAITDSELSDEEASILESGGFSVSRATTPQLTDVSEDLDQQSLPSEPEETLSRDLGEGEEGELAPPEDLLGRPQALSRQALDSEEEEEDVAAKETLLRLSSPLHFVNTHFNGAGSPPDGVKCSPGGPVETLSPETVSGGLTALPGTLSPPLCLVGSDPAPSPSILPPVPQDSPQPLPAPEEEEALTTEDFELLDQGELEQLNAELGLEPETPPKPPDAPPLGPDIHSLVQSDQEAQAVAEP

• Full Name: family with sequence similarity 134, member A
• Calculated Molecular Weight: 543 aa, 58 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC002420
• Gene Symbol: FAM134A
• Gene ID (NCBI): 79137
• RRID: AB_3085745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NC44
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.