FAM134A Recombinant antibody

• 

  WB analysis using 85791-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85791-4-RR (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 85791-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 85791-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85791-4-RR (FAM134A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85791-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85791-4-RR against Human FAM134A were performed. The affinity constant is 3.17 nM.

FAM134A Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85791-4-RR

Clone No.250097C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C2orf17, MAG2, Protein FAM134A, Reticulophagy regulator 2, RETREG2

---

Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, U2OS cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85791-4-RR targets FAM134A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FAM134A fusion protein Ag20025
• Full Name: family with sequence similarity 134, member A
• Calculated Molecular Weight: 543 aa, 58 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC002420
• Gene Symbol: FAM134A
• Gene ID (NCBI): 79137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NC44
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.