Validation Data Gallery
|Positive WB detected in||C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum|
|Positive IHC detected in||mouse brain tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
21537-1-AP targets FAM134B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FAM134B fusion protein Ag16176|
|Full Name||family with sequence similarity 134, member B|
|Calculated molecular weight||497 aa, 55 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC030517|
|Gene ID (NCBI)||54463|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).
Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.
Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.
Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development
Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
ATF4 links ER stress with reticulophagy in glioblastoma cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-06-2020)
The antibody works well. There were a few non-specific bands in HEK293T lysates though.