FAM134B Polyclonal antibody

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  WB analysis of HEK-293 using 21537-1-AP

  WB result of FAM134B antibody (21537-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM134B transfected HEK-293 cells.

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  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21537-1-AP

  IP result of anti-FAM134B (IP:21537-1-AP, 4ug; Detection:21537-1-AP 1:5000) with Jurkat cells lysate 1600 ug.

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  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

FAM134B Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21537-1-AP

Publications(49)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1, JK1

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Tested Applications

• Positive WB detected in: C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 42 publications below
• IHC: See 1 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Product Information

21537-1-AP targets FAM134B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM134B fusion protein Ag16176
• Full Name: family with sequence similarity 134, member B
• Calculated Molecular Weight: 497 aa, 55 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC030517
• Gene Symbol: FAM134B
• Gene ID (NCBI): 54463
• RRID: AB_2878879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H6L5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
human	WB,IF	Nat Commun; AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity; Authors - Linliang Zhang
human	WB	Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham ; KD Validated
human	WB	Nat Commun; Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.; Authors - Mark L Schultz
mouse	WB,IF	Dev Cell; Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development; Authors - Patrick Lüningschrör
human	WB	Autophagy; Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.; Authors - Jonathan A Stefely

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (04-06-2020)

The antibody works well. There were a few non-specific bands in HEK293T lysates though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FAM134B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21537-1-AP)