|Positive WB detected in||rat brain tissue, NIH/3T3 cells, HEK-293 cells, mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21537-1-AP is FAM134B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FAM134B fusion protein Ag16176|
|Full Name||family with sequence similarity 134, member B|
|Calculated molecular weight||497 aa, 55 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC030517|
|Gene ID (NCBI)||54463|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.
Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
J Biol Chem
Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.
Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.
ATF4 links ER stress with reticulophagy in glioblastoma cells.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-06-2020)
The antibody works well. There were a few non-specific bands in HEK293T lysates though.