FAM134B Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM134B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 21537-1-AP

Synonyms

FAM134B, Protein FAM134B, Reticulophagy receptor 1, RETREG1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 21537-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21537-1-AP

  WB result of FAM134B antibody (21537-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM134B transfected HEK-293 cells.

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  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21537-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat brain tissue, NIH/3T3 cells, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below

Product Information

The immunogen of 21537-1-AP is FAM134B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM134B fusion protein Ag16176
• Full Name: family with sequence similarity 134, member B
• Calculated molecular weight: 497 aa, 55 kDa
• Observed molecular weight: 65 kDa
• GenBank accession number: BC030517
• Gene symbol: FAM134B
• Gene ID (NCBI): 54463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
		Mol Cell; TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.; Authors - Heeseon An
human	WB	Mol Cell; Intrinsically Disordered Protein TEX264 Mediates ER-phagy.; Authors - Haruka Chino
human	WB	J Biol Chem; Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.; Authors - Yangjie Liao
human	WB	Autophagy; Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.; Authors - Nicholas J Lennemann
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke ; KO Validated
human	WB	EMBO J; CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.; Authors - Thaddaeus Mutugi Nthiga

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-06-2020)

The antibody works well. There were a few non-specific bands in HEK293T lysates though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FAM134B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21537-1-AP)