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  • KD/KO Validated

FAM134B Polyclonal antibody

FAM134B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21537-1-AP

Synonyms

FAM134B, Protein FAM134B, Reticulophagy receptor 1, RETREG1



Tested Applications

Positive WB detected inrat brain tissue, NIH/3T3 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21537-1-AP is FAM134B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM134B fusion protein Ag16176
Full Name family with sequence similarity 134, member B
Calculated molecular weight 497 aa, 55 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC030517
Gene symbol FAM134B
Gene ID (NCBI) 54463
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM134B antibody 21537-1-APDownload protocol
IHC protocol for FAM134B antibody 21537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

Authors - Heeseon An
humanWB

Mol Cell

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Authors - Haruka Chino
humanWB,IF

Cell Death Dis

Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway.

Authors - Luchao Li
humanWB

J Biol Chem

Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.

Authors - Yangjie Liao
humanWB

Autophagy

Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.

Authors - Nicholas J Lennemann
humanWB

Autophagy

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Authors - Svenja Zielke
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-06-2020)

The antibody works well. There were a few non-specific bands in HEK293T lysates though.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FAM134B Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21537-1-AP)