- Featured Product
- KD/KO Validated
FAM134B Polyclonal antibody
FAM134B Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21537-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, NIH/3T3 cells, HEK-293 cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
Product Information
The immunogen of 21537-1-AP is FAM134B Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FAM134B fusion protein Ag16176 |
Full Name | family with sequence similarity 134, member B |
Calculated molecular weight | 497 aa, 55 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC030517 |
Gene symbol | FAM134B |
Gene ID (NCBI) | 54463 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FAM134B antibody 21537-1-AP | Download protocol |
IHC protocol for FAM134B antibody 21537-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress. | ||
J Biol Chem Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells. | ||
Autophagy Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH H (Verified Customer) (04-06-2020) | The antibody works well. There were a few non-specific bands in HEK293T lysates though.
![]() |