FAM135B Polyclonal antibody

FAM135B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28061-1-AP

Synonyms

C8ORFK32, FAM135B, Protein FAM135B

---

Validation Data Gallery

• 

  WB analysis using 28061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28061-1-AP (FAM135B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 28061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 28061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 28061-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 28061-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: C6 cells, Neuro-2a cells
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28061-1-AP targets FAM135B in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, mouse samples.

• Tested Reactivity: Human, Rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM135B fusion protein Ag27897
• Full Name: family with sequence similarity 135, member B
• Observed Molecular Weight: 126-155 kDa
• GenBank Accession Number: BC106912
• Gene Symbol: FAM135B
• Gene ID (NCBI): 51059
• RRID: AB_2881050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.