FAM13A Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM13A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 55401-1-AP

Synonyms

FAM13A, FAM13A1, KIAA0914, Protein FAM13A

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 55401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 55401-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 55401-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 55401-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55401-1-AP (FAM13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsil using 55401-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 55401-1-AP (FAM13A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55401-1-AP

  Immunofluorescent analysis of HeLa cells using 55401-1-AP (FAM13A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse lung tissue, mouse ovary tissue, mouse pancreas tissue
• Positive IHC detected in: human skeletal muscle tissue, human tonsil tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

55401-1-AP targets FAM13A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: family with sequence similarity 13, member A
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_014883
• Gene Symbol: FAM13A
• Gene ID (NCBI): 10144
• RRID: AB_11182723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM13A, also named as FAM13A1 and KIAA0914, belongs to the FAM13 family. It is associated with chronic obstructive pulmonary disease.(PMID: 20173748) This antibody is specific to FAM13A. It can recognize all the isoforms of FAM13A.

Publications

Species	Application	Title
mouse	WB	J Immunol; Annexin A2 Regulates Autophagy in Pseudomonas aeruginosa Infection through the Akt1-mTOR-ULK1/2 Signaling Pathway.; Authors - Rongpeng Li
	WB, IHC	Front Physiol; A Protective Role of FAM13A in Human Airway Epithelial Cells Upon Exposure to Cigarette Smoke Extract.; Authors - Qing Chen
human	WB	Mol Med Rep; miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia.; Authors - Enbo Wang ; KD Validated
human	IHC	Am J Physiol Lung Cell Mol Physiol; FAM13A regulates cellular senescence marker p21 and mitochondrial reactive oxygen species production in airway epithelial cells; Authors - Qing Chen
human	IF	Am J Respir Cell Mol Biol; AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex; Authors - Lu Gong
human	WB	Am J Respir Cell Mol Biol; The FAM13A Long Isoform Regulates Cilia Movement and Co-ordination in Airway Mucociliary Transport; Authors - Ashleigh Howes