FAM158A Polyclonal antibody

FAM158A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23919-1-AP

Synonyms

C14orf122, CGI 112, FAM158A, UPF0172 protein FAM158A

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Validation Data Gallery

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  WB analysis of HEK-293 using 23919-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23919-1-AP (FAM158A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 23919-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23919-1-AP (FAM158A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23919-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23919-1-AP (FAM158A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 23919-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23919-1-AP (FAM158A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23919-1-AP is FAM158A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM158A fusion protein Ag20744
• Full Name: family with sequence similarity 158, member A
• Calculated Molecular Weight: 208 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC002491
• Gene Symbol: FAM158A
• Gene ID (NCBI): 51016
• RRID: AB_2879361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UPF0172 protein FAM158A, also known as c14orf122 or CGI112, is a protein that in humans is encoded by the FAM158A gene located on chromosome 14q11.2. Human FAM158A and its paralogs in other species are part of the uncharacterized protein family UPF0172 family, which is a subset of the JAB1/Mov34/MPN/PAD-1 ubiquitin protease protein family. The MPN superfamily contributes to ubiquitination and de-ubiquitination activity within the cell. The UPF0172 subset no longer has a functional ubiquitination domain and the function is uncharacterized. Fam158a is nearly ubiquitously expressed throughout the human body. The homolog in mice also shows expression throughout the entire body. Several micro-arrays demonstrate the variable expression of Fam158a in response to other factors and in various cancer types. None of this information gives any indication of a specific function but the wide expression of the gene and its high conservation indicate that Fam158a plays an important role in cellular function.