FAM171A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM171A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20836-1-AP

Synonyms

Protein FAM171A2

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Validation Data Gallery

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  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20836-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20836-1-AP targets FAM171A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM171A2 fusion protein Ag14909
• Full Name: family with sequence similarity 171, member A2
• Calculated Molecular Weight: 826 aa, 87 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC030200
• Gene Symbol: FAM171A2
• Gene ID (NCBI): 284069
• RRID: AB_2878748
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM171A2 is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases. In addition, FAM171A2 was considerably expressed in the vascular endothelium and microglia, which are rich in Progranulin (PGRN) (PMID: 33087363).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Sci Adv; The FAM171A2 gene is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases.; Authors - Wei Xu ; KD Validated