FAM175B Polyclonal antibody

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  WB analysis of HL-60 using 26868-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26868-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26868-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26868-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26868-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM175B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26868-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Abraxas brother protein 1, ABRAXAS2, ABRO1, BRISC complex subunit Abraxas 2, BRISC complex subunit Abro1

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Tested Applications

• Positive WB detected in: HL-60 cells, HEK-293 cells, mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26868-1-AP targets FAM175B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM175B fusion protein Ag22121
• Full Name: family with sequence similarity 175, member B
• Calculated Molecular Weight: 415 aa, 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008999
• Gene Symbol: FAM175B
• Gene ID (NCBI): 23172
• RRID: AB_2880663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15018
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM175B (also known as ABRO1/KIAA0157) is a scaffold subunit of the BRCC36-containing deubiquitinase complex (BRISC) that specifically cleaves Lys63-linked polyubiquitin chains, thereby modulating DNA-damage responses and receptor trafficking. Originally identified as a binding partner of JunD and ATF4, FAM175B stabilizes ATF4 by inhibiting its ubiquitin-dependent degradation, leading to activation of the ATF4-CHOP pathway and induction of apoptosis under endoplasmic reticulum stress. In esophageal squamous cell carcinoma, FAM175B expression is down-regulated and acts as a p53-independent tumor suppressor; restoring its expression reduces proliferation and increases apoptosis of p53-mutated tumor cells. Collectively, FAM175B serves as a critical scaffold that links deubiquitinase activity to transcriptional and pro-apoptotic programs, offering a potential biomarker for early diagnosis and therapeutic targeting in cancers with p53 dysfunction (PMID: 30854784).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Autophagy; A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response; Authors - Xinming Pan