Tested Applications
| Positive IHC detected in | human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25395-1-AP targets FAM20C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22061 Product name: Recombinant human FAM20C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 121-269 aa of BC040074 Sequence: AHRPLLRDPGPRRSESPPGPGGDASLLARLFEHPLYRVAVPPLTEEDVLFNVNSDTRLSPKAAENPDWPHAGAEGAEFLSPGEAAVDSYPNWLKFHIGINRYELYSRHNPAIEALLHDLSSQRITSVAMKSGGTQLKLIMTFQNYGQAL Predict reactive species |
| Full Name | family with sequence similarity 20, member C |
| Calculated Molecular Weight | 584 aa, 66 kDa |
| GenBank Accession Number | BC040074 |
| Gene Symbol | FAM20C |
| Gene ID (NCBI) | 56975 |
| RRID | AB_2880058 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM20C, also named as Golgi-enriched fraction casein kinase or Dentin matrix protein 4, is a 584 amino acid protein, which belongs to the FAM20 family. FAM20C is widely expressed. FAM20C as a Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM20C antibody 25395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-FAM20C antibody (Cat# 25395-1-AP) has 9 total citations published in journals including Nature Communications, Journal of Clinical Investigation, JCI Insight, International Journal of Biological Macromolecules, Cell Proliferation, Frontiers in Immunology, FASEB Journal, and Gene. Research fields span bone dysplasia, obesity-related insulin resistance, triple-negative breast cancer, lung squamous cell carcinoma, cardiovascular disease, liver fibrogenesis, and bone development.
| Species | Application | Title |
|---|---|---|
Nat Commun Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia | ||
J Clin Invest Secretory kinase FAM20C triggers adipocyte dysfunction, inciting insulin resistance and inflammation in obesity.
| ||
Int J Biol Macromol Discovery of a novel Fam20C inhibitor for treatment of triple-negative breast cancer | ||
Cell Prolif Chromatin Remodeller BRD9 Orchestrates Odontoblastic Differentiation via Coordinating RUNX2-KLF4. | ||
JCI Insight C-terminal FGF-23 production coupling with aldosterone via FAM20C and predicting cardiovascular events in primary aldosteronism
|
Reviews
What customers say
The single review for this antibody is strongly negative. The reviewer (Doug Chan, May 2019) rated it 1 star and stated it "does not work," calling it "garbage" and advising others not to waste their money. They used a 1:1000 dilution for Western Blot on MDA-MB-231 cells, a line in which they could readily detect FAM20C by mass spectrometry, yet the antibody failed to produce a signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Doug (Verified Customer) (05-01-2019) | Does not work. This antibody is garbage. Don't waste your money.
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