FAM21C Polyclonal antibody

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  WB analysis using 33047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33047-1-AP (FAM21C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 33047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FAM21C antibody (33047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM21C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HuH-7 cells, L02 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33047-1-AP targets FAM21C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15066

  Product name: Recombinant human FAM21C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1036-1318 aa of BC006456

  Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ

• Full Name: family with sequence similarity 21, member C
• Calculated Molecular Weight: 1320 aa, 145 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC006456
• Gene Symbol: FAM21C
• Gene ID (NCBI): 253725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y4E1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.