FAM21C Polyclonal antibody

• Home
• Products
• Primary Antibodies
• FAM21C Polyclonal antibody (33047-1-AP)

• 

  WB analysis using 33047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33047-1-AP (FAM21C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 33047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FAM21C antibody (33047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM21C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HuH-7 cells, L02 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33047-1-AP targets FAM21C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15066

  Product name: Recombinant human FAM21C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1036-1318 aa of BC006456

  Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ

• Full Name: family with sequence similarity 21, member C
• Calculated Molecular Weight: 1320 aa, 145 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC006456
• Gene Symbol: FAM21C
• Gene ID (NCBI): 253725
• RRID: AB_3742836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y4E1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASHC2C encodes WASH complex subunit 2C, a core subunit of the WASH complex that organizes endosomal membrane remodeling and cargo recycling. It binds phospholipids such as PtdIns-(4)P and the retromer component VPS35, linking endosomal localization to actin-based membrane dynamics. The protein is found on the early endosome membrane, in the early endosome, endosome, and cytosol, and is expressed at high levels in the male reproductive system with detectable expression in brain, endocrine system, and hematopoietic system.