FAM49B Polyclonal antibody

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  WB analysis of MCF-7 using 20127-1-AP

  WB result of FAM49B antibody (20127-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM49B transfected MCF-7 cells.

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  WB analysis of human placenta using 20127-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 20127-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20127-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20127-1-AP

  IP result of anti-FAM49B (IP:20127-1-AP, 4ug; Detection:20127-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 20127-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20127-1-AP

  Immunofluorescent analysis of HepG2 cells, using FAM49B antibody 20127-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

FAM49B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20127-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

BM 009, FAM49B, L1, Protein FAM49B

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse brain tissue, human placenta tissue, human testis tissue, mouse testis tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below

Product Information

20127-1-AP targets FAM49B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM49B fusion protein Ag13939
• Full Name: family with sequence similarity 49, member B
• Calculated Molecular Weight: 324 aa, 37 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC003599
• Gene Symbol: FAM49B
• Gene ID (NCBI): 51571
• RRID: AB_10638431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUQ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
monkey	WB	Nat Cell Biol; Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.; Authors - Loic Fort ; KD Validated
human	WB	Genes (Basel); Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.; Authors - Yijian Chen ; KO Validated
mouse	WB	Mol Immunol; FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.; Authors - Zuotian Huang ; KD Validated
		Elife; CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake; Authors - Savvas Nikolaou