FAM65B Polyclonal antibody

• Featured Product
• KD/KO Validated

FAM65B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17015-1-AP

Synonyms

C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17015-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17015-1-AP (FAM65B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

17015-1-AP targets FAM65B in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM65B fusion protein Ag9991
• Full Name: family with sequence similarity 65, member B
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 115 kDa, 66 kDa
• GenBank Accession Number: BC001232
• Gene Symbol: FAM65B
• Gene ID (NCBI): 9750
• RRID: AB_10640810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.

Publications

Species	Application	Title
mouse, rat	WB,IF	Proc Natl Acad Sci U S A; FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.; Authors - Oscar Diaz-Horta
mouse	WB	J Cell Sci; Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.; Authors - Kun Gao ; KO Validated
human	WB	FASEB J; Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.; Authors - Anuradha Balasubramanian