FAM83A Polyclonal antibody

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• FAM83A Polyclonal antibody (20618-1-AP)

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• KD/KO Validated

FAM83A Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 20618-1-AP Copied!

Publications (20)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

BJ TSA 9, Protein FAM83A, TSGP, Tumor antigen BJ TSA 9, Tumor antigen BJ-TSA-9

• 

  WB analysis using 20618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20618-1-AP (FAM83A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 20618-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20618-1-AP (FAM83A Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 20618-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20618-1-AP (FAM83A Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 20618-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FAM83A Polyclonal antibody (20618-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: BxPC-3 cells, DU 145 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20618-1-AP targets FAM83A in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14680

  Product name: Recombinant human FAM83A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-294 aa of BC007828

  Sequence: MSRSRHLGKIRKRLEDVKSQWVRPARADFSDNESARLATDALLDGGSEAYWRVLSQEGEVDFLSSVEAQYIQAQAREPPCPPDTLGGAEAGPKGLDSSSLQSGTYFPVASEGSEPALLHSWASAEKPYLKEKSSATVYFQTVKHNNIRDLVRRCITRTSQNISIRSVEGEIYCAKSGRKFTGQIREKFIISDWRFVLSGSYSFTWLCGHVHRNILSKFTGQAVELFDEEFRHLYASSKPVMGLKSPRLVAPVPPGAAPANGRLSSSSGSASDRTSSNPFSGRSAGSHPGTRTDG

• Full Name: family with sequence similarity 83, member A
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 47-58 kDa
• GenBank Accession Number: BC007828
• Gene Symbol: FAM83A
• Gene ID (NCBI): 84985
• RRID: AB_2878708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UY5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-FAM83A antibody (Cat# 20618-1-AP) has 20 citations spanning journals including Biomarkers Research, Cell Death Discovery, Journal of Translational Medicine, Clinical Epigenetics, Cell Oncology, International Journal of Molecular Sciences, Lung Cancer, Scientific Reports, BMC Cancer, and Discover Oncology. Research fields cover oncology (lung, breast, pancreatic, ovarian, gastric, and liver cancers), ferroptosis, Wnt/β-catenin signaling, epithelial-mesenchymal transition, epigenetics, and microRNA regulation.

Automatically generated from the 20 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Biomark Res; FAM83A is a potential biomarker for breast cancer initiation.; Authors - Natascia Marino
human	WB	Cell Death Discov; Family with sequence similarity 83, member A (FAM83A) inhibits ferroptosis via the Wnt/β-catenin pathway in lung squamous cell cancer; Authors - Cong Wang ; KD Validated
human	WB,IHC	J Transl Med; FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma.; Authors - Huan Ji ; KD Validated
human	IHC	Clin Epigenetics; Aberrant epigenetic and transcriptional events associated with breast cancer risk.; Authors - Natascia Marino
human	IHC	Cell Oncol (Dordr); Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer; Authors - Guangyu Chen
human	WB	Int J Mol Sci; Involvement of MicroRNA-1-FAM83A Axis Dysfunction in the Growth and Motility of Lung Cancer Cells.; Authors - Pei-Jung Liu ; KD Validated