Tested Applications
| Positive WB detected in | MCF-7 cells, Jurkat cells, MDA-MB-453s cells |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:7000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18421-1-AP targets FAM84B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13243 Product name: Recombinant human FAM84B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC052957 Sequence: MGNQVEKLTHLSYKEVPTADPTGVDRDDGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPRLHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQAQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLSSSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQPYRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAEPEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH Predict reactive species |
| Full Name | family with sequence similarity 84, member B |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC052957 |
| Gene Symbol | FAM84B |
| Gene ID (NCBI) | 157638 |
| RRID | AB_2278161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM84B antibody 18421-1-AP | Download protocol |
| IP protocol for FAM84B antibody 18421-1-AP | Download protocol |
| WB protocol for FAM84B antibody 18421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FAM84B antibody (Cat# 18421-1-AP) has accumulated 14 citations across journals including Nature Genetics, PNAS, Cell Death & Disease, Cell Discovery, Oncotarget, Aging, Gigascience, Scientific Reports, Pathology Research & Practice, and Cancer Science. Associated research fields encompass oncology (cervical, prostate, lung, esophageal, pancreatic, breast, and gastric cancers), vesicular trafficking, DNA methylation and epigenetics, and tumor-infiltrating lymphocyte immunotherapy.
| Species | Application | Title |
|---|---|---|
Nat Genet Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism. | ||
Cell Mol Biol Lett Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis | ||
Cell Death Discov Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma. | ||
Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.
| ||
Aging (Albany NY) FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway. |
