FAM92B/CIBAR2 Polyclonal antibody

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  WB analysis of HeLa using 26413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 26413-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 26413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 26413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26413-1-AP ( FAM92B/CIBAR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse lung using 26413-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FAM92B/CIBAR2 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 26413-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

FAM92B, Protein FAM92B, CIBAR2, CBY1-interacting BAR domain-containing protein 2

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, mouse small intestine tissue
• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

26413-1-AP targets FAM92B/CIBAR2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM92B/CIBAR2 fusion protein Ag24802
• Full Name: family with sequence similarity 92, member B
• Calculated Molecular Weight: 304 aa, 35 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC111944
• Gene Symbol: FAM92B
• Gene ID (NCBI): 339145
• RRID: AB_2880506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZTR7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM92B (family with sequence similarity 92, member B, also named CIBAR2) is a transmembrane protein that plays a crucial role in various cellular processes, including membrane trafficking and cell adhesion. The protein is predicted to be intracellular and is involved in ciliogenesis, likely through the recruitment and fusion of endosomal vesicles at distal appendages during early stages of ciliogenesis.

Publications

Species	Application	Title
mouse	IF	Life Sci Alliance; ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance; Authors - Eunice N Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eunice (Verified Customer) (10-24-2022)

This was tested on mouse tracheal epithelial cells fixed in 4% PFA overnight and on MEFs fixed for 10 min in ice-cold methanol acetone. Works well.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Tracheal epithelial cells, mouse embryonic fibroblasts (MEFs)