FANCA Polyclonal antibody

• Featured Product
• KD/KO Validated

FANCA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11975-1-AP

Synonyms

FA, FA H, FA1, FAA, FACA, FAH, FANCA, FANCH, Fanconi anemia group A protein, Protein FACA

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Validation Data Gallery

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  WB analysis of HeLa cells using 11975-1-AP

  WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells.

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  WB analysis using 11975-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11975-1-AP targets FANCA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCA fusion protein Ag2598
• Full Name: Fanconi anemia, complementation group A
• Calculated Molecular Weight: 163 kDa
• Observed Molecular Weight: 163 kDa
• GenBank Accession Number: BC008979
• Gene Symbol: FANCA
• Gene ID (NCBI): 2175
• RRID: AB_2101786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu ; KO Validated
mouse	WB	EMBO Mol Med; WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.; Authors - Amanpreet Kaur
human	WB	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	IHC,IF,IP	Gene; Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing.; Authors - Yu Feng