FANCC Polyclonal antibody

FANCC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16973-1-AP

Synonyms

FA3, FAC, FACC, FANCC, Fanconi anemia group C protein, Protein FACC

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Validation Data Gallery

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  WB analysis of HepG2 using 16973-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16973-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16973-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16973-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16973-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, K-562 cells, Jurkat cells, NIH/3T3 tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16973-1-AP targets FANCC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCC fusion protein Ag9423
• Full Name: Fanconi anemia, complementation group C
• Calculated Molecular Weight: 558 aa, 63 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC015748
• Gene Symbol: FANCC
• Gene ID (NCBI): 2176
• RRID: AB_2231524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

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