Phospho-FANCD2 (Ser330) Polyclonal antibody

Phospho-FANCD2 (Ser330) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19543-1-AP

Synonyms

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2

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Validation Data Gallery

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  WB analysis of HL-60 using 19543-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19543-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19543-1-AP targets Phospho-FANCD2 (Ser330) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Fanconi anemia, complementation group D2
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 150-155 kDa
• GenBank Accession Number: NM_033084
• Gene Symbol: FANCD2
• Gene ID (NCBI): 2177
• RRID: AB_10640576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is to phospho-Ser(330) of FANCD2.