Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28619-1-AP targets FANCD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29460 Product name: Recombinant human FANCD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of NM_001018115 Sequence: MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASM Predict reactive species |
| Full Name | Fanconi anemia, complementation group D2 |
| Calculated Molecular Weight | 164 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_001018115 |
| Gene Symbol | FANCD2 |
| Gene ID (NCBI) | 2177 |
| RRID | AB_2881182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXW9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for FANCD2 antibody 28619-1-AP | Download protocol |
| WB protocol for FANCD2 antibody 28619-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FANCD2 Polyclonal antibody (Cat# 28619-1-AP) has 14 citations published in journals including Nature Communications, Advanced Science, Cell Death & Disease, Cancer Letters, Phytomedicine, Acta Pharmacologica Sinica, ACS Applied Materials & Interfaces, Cell Reports, European Journal of Pharmacology, Frontiers in Pharmacology, iScience, and Journal of Gastrointestinal Oncology. Research fields span DNA repair, the Fanconi Anemia pathway, cancer biology (hepatocellular carcinoma, glioblastoma, cervical cancer, esophageal cancer, CML, lung adenocarcinoma), ferroptosis, cuproptosis, and drug resistance mechanisms.
| Species | Application | Title |
|---|---|---|
Nat Commun Acetylation-dependent regulation of core spliceosome modulates hepatocellular carcinoma cassette exons and sensitivity to PARP inhibitors | ||
Adv Sci (Weinh) HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway | ||
Adv Sci (Weinh) The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability | ||
Cell Death Dis Therapeutic targeting of ARID1A-deficient cancer cells with RITA (Reactivating p53 and inducing tumor apoptosis) | ||
Phytomedicine Celastrol induces DNA damage and cell death in BCR-ABL T315I-mutant CML by targeting YY1 and HMCES |
Reviews
What customers say
The single review for this product is a 5-star rating. The user tested it on U2OS human osteosarcoma cells for Western Blot, Immunofluorescence, and cell culture at a 1:1000 dilution. They reported that the antibody worked perfectly for detecting both overexpressed and endogenous protein, with no background signal. Overall, the feedback is highly positive, highlighting strong specificity and clean results across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexandra (Verified Customer) (07-12-2024) | Work perfectly both on overexpressed and endogenous protein. No background
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