FANCD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FANCD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 28619-1-AP

Synonyms

DKFZp762A223, FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, FLJ23826, Protein FACD2

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Validation Data Gallery

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  WB analysis using 28619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28619-1-AP (FANCD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

28619-1-AP targets FANCD2 in WB, IP, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCD2 fusion protein Ag29460
• Full Name: Fanconi anemia, complementation group D2
• Calculated Molecular Weight: 164 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001018115
• Gene Symbol: FANCD2
• Gene ID (NCBI): 2177
• RRID: AB_2881182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies.

Publications

Species	Application	Title
human	WB	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou
human	WB,IP	Front Pharmacol; Abnormally Expressed Ferroptosis-Associated FANCD2 in Mediating the Temozolomide Resistance and Immune Response in Glioblastoma.; Authors - Liying Song ; KD Validated
human	IF	Eur J Pharmacol; Rapid DNA interstrand cross-linking of Pt(IV) compound.; Authors - Chun-Lai Zhao
human	WB	Adv Sci (Weinh); HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway; Authors - Zixian Huang