FANCI Polyclonal antibody

FANCI Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20789-1-AP

Synonyms

Protein FACI, Fanconi anemia group I protein

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Validation Data Gallery

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  WB analysis using 20789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20789-1-AP (FANCI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20789-1-AP

  IP result of anti-FANCI (IP:20789-1-AP, 4ug; Detection:20789-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 20789-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 20789-1-AP (FANCI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

20789-1-AP targets FANCI in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCI fusion protein Ag14386
• Full Name: Fanconi anemia, complementation group I
• Calculated Molecular Weight: 1328 aa, 149 kDa
• Observed Molecular Weight: 149 kDa
• GenBank Accession Number: BC004277
• Gene Symbol: FANCI
• Gene ID (NCBI): 55215
• RRID: AB_10694829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; Aberrant R-loop-mediated immune evasion, cellular communication, and metabolic reprogramming affect cancer progression: a single-cell analysis; Authors - Shichao Zhang
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB,CoIP	Cell Death Dis; Inactivation of ribosomal protein S27-like impairs DNA interstrand cross-link repair by destabilization of FANCD2 and FANCI.; Authors - Siyuan Sun
human		Adv Sci (Weinh); Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic Functions; Authors - Tangming Guan
	WB	Mol Carcinog; KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2; Authors - Lei Zhong