FANCI Monoclonal antibody

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• FANCI Monoclonal antibody (67304-1-Ig)

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FANCI Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67304-1-Ig Copied!

Clone No. 1B10E6

Publications (3)

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Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA
For FC, select conjugated version.

Synonyms

Fanconi anemia group I protein, Protein FACI

• 

  WB analysis of HeLa using 67304-1-Ig

  WB result of FANCI antibody (67304-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCI transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 67304-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 67304-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, LNCaP cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, Daudi cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15153

  Product name: Recombinant human FANCI protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-252 aa of BC004277

  Sequence: MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIVSEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDE

• Full Name: Fanconi anemia, complementation group I
• Calculated Molecular Weight: 1328 aa, 149 kDa
• Observed Molecular Weight: 149 kDa, 142 kDa
• GenBank Accession Number: BC004277
• Gene Symbol: FANCI
• Gene ID (NCBI): 55215
• RRID: AB_2882566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NVI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Antibody 67304-1-Ig (anti-FANCI) has 3 total citations, published in Advanced Science (2 papers) and Frontiers in Immunology (1 paper). Research fields include the Fanconi Anemia DNA repair pathway, head and neck squamous cell carcinoma chemosensitization, replication stress-induced genomic instability, and skin cutaneous melanoma prognosis. All studies used human samples with WB and/or IHC applications.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway; Authors - Zixian Huang
human	WB	Adv Sci (Weinh); The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability; Authors - Pengqing Nie
human	WB,IHC	Front Immunol; FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma; Authors - Zhenguo Cai