FANCM Polyclonal antibody

FANCM Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12954-1-AP

Synonyms

FAAP250, FANCM, Fanconi anemia group M protein, KIAA1596, Protein FACM, Protein Hef ortholog

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Validation Data Gallery

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  WB analysis of HeLa using 12954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12954-1-AP (FANCM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12954-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12954-1-AP (FANCM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12954-1-AP targets FANCM in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCM fusion protein Ag3618
• Full Name: Fanconi anemia, complementation group M
• Calculated Molecular Weight: 2048 aa, 232 kDa
• Observed Molecular Weight: 250 kDa, 232 kDa
• GenBank Accession Number: BC036056
• Gene Symbol: FANCM
• Gene ID (NCBI): 57697
• RRID: AB_10644146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	EMBO J; m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region.; Authors - Zhihao Wang
human	WB	Onco Targets Ther; The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer.; Authors - Xiaofei Wang