FANCM Polyclonal antibody

FANCM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12954-1-AP

Synonyms

KIAA1596, Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13

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Validation Data Gallery

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  WB analysis of HeLa using 12954-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12954-1-AP (FANCM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

12954-1-AP targets FANCM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCM fusion protein Ag3618
• Full Name: Fanconi anemia, complementation group M
• Calculated Molecular Weight: 2048 aa, 232 kDa
• Observed Molecular Weight: 250 kDa, 232 kDa
• GenBank Accession Number: BC036056
• Gene Symbol: FANCM
• Gene ID (NCBI): 57697
• RRID: AB_10644146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCM (Fanconi anemia group M protein) is a DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PMID: 16116422). It is required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PMID:16116422; 19423727; 20347428). FANCM is localized in the cell nucleus and can bind double-stranded DNA (dsDNA), fork-structured DNA (fsDNA), and Holliday junction substrates (PMID: 20347428; 20347429).

Publications

Species	Application	Title
mouse	WB	EMBO J; m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region.; Authors - Zhihao Wang
human	WB	Onco Targets Ther; The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer.; Authors - Xiaofei Wang
human	WB	mSystems; Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction; Authors - Wei Hu
mouse	WB	Cell Prolif; Lin28a forms an RNA-binding complex with Igf2bp3 to regulate m6A-modified stress response genes in stress granules of muscle stem cells; Authors - Dan Song