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FANCM Recombinant monoclonal antibody, PBS Only (82897-2-PBS)

FANCM Recombinant monoclonal antibody, PBS Only

Name: FANCM Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 82897-2-PBS-100UG

FANCM Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 82897-2-PBS

Clone No. 230233G5

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

230233G5, ATP-dependent RNA helicase FANCM, EC:3.6.4.13, FAAP250, Fanconi anemia group M protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

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Filter: AllIPIHCIF/ICCFC (Intra)

IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)
IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug. This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)
1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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Product Information

82897-2-PBS targets FANCM in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag34447 Product name: Recombinant human FANCM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1481-1679 aa of BC140776 Sequence: VARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCL Predict reactive species
Full Name	Fanconi anemia, complementation group M
GenBank Accession Number	BC140776
Gene Symbol	FANCM
Gene ID (NCBI)	57697
RRID	AB_3670625
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8IYD8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.