FARP1 Recombinant antibody

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  WB analysis using 85638-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85638-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85638-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85638-4-RR against Human FARP1 were performed. The affinity constant is 1.18 nM.

FARP1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85638-4-RR

Clone No.243132G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CDEP, Chondrocyte-derived ezrin-like protein, FERM, ARHGEF and pleckstrin domain-containing protein 1, FERM, RhoGEF and pleckstrin domain-containing protein 1, PH domain-containing family C member 2

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, HeLa cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85638-4-RR targets FARP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FARP1 fusion protein Ag21225
• Full Name: FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
• Calculated Molecular Weight: 1076 aa, 122 kDa
• Observed Molecular Weight: 122-130 kDa
• GenBank Accession Number: BC071592
• Gene Symbol: FARP1
• Gene ID (NCBI): 10160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4F1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.