FARSA Polyclonal antibody

• Featured Product
• KD/KO Validated

FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18121-1-AP

Synonyms

Phenylalanine--tRNA ligase alpha subunit, FARSLA, FARSL, FARS, EC:6.1.1.20

---

Validation Data Gallery

• 

  WB analysis using 18121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18121-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18121-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 18121-1-AP

  IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.

• 

  IHC staining of human liver cancer using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18121-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 18121-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of A431 using 18121-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (18121-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 18121-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

18121-1-AP targets FARSA in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FARSA fusion protein Ag12647
• Full Name: phenylalanyl-tRNA synthetase, alpha subunit
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006495
• Gene Symbol: FARSA
• Gene ID (NCBI): 2193
• RRID: AB_2102509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Clin Genet; FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!; Authors - Luise A Schuch
human,rat,mouse	IF,ELISA	Nat Commun; Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.; Authors - Qian Zhou ; KO Validated
human	WB,IP	Autoimmun Rev; Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase; Authors - Yoshinao Muro
human,mouse	WB,IF,RIP	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj