FARSB Polyclonal antibody

FARSB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16341-1-AP

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 16341-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 16341-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16341-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HL-60 using 16341-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 16341-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human liver using 16341-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 16341-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16341-1-AP (FARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 16341-1-AP
IP result of anti-FARSB (IP:16341-1-AP, 4ug; Detection:16341-1-AP 1:500) with HeLa cells lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16341-1-AP (FARSB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 16341-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 16341-1-AP (FARSB antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 16341-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16341-1-AP (FARSB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for FARSB in ?

Overview

Tested Applications
Product Information
Publications (5)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, HL-60 cells, K-562 cells, human liver tissue, mouse brain tissue, PC-3 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, mouse cerebellum tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16341-1-AP targets FARSB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9522 Product name: Recombinant human FARSB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-589 aa of BC017783 Sequence: DHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINIGPFL Predict reactive species
Full Name	phenylalanyl-tRNA synthetase, beta subunit
Calculated Molecular Weight	589 aa, 66 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC017783
Gene Symbol	FARSB
Gene ID (NCBI)	10056
RRID	AB_2102515
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NSD9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for FARSB antibody 16341-1-AP	Download protocol
IHC protocol for FARSB antibody 16341-1-AP	Download protocol
IP protocol for FARSB antibody 16341-1-AP	Download protocol
WB protocol for FARSB antibody 16341-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The FARSB polyclonal antibody (Cat# 16341-1-AP) has 5 citations. These appear in Nature Communications (2), Journal of the American Chemical Society, Neural Regeneration Research, and International Journal of Molecular Sciences. The research fields span neurodegeneration (resveratrol effects on histone modification), ALS/FTD-associated RNA toxicity, bioorthogonal chemistry, peripheral nerve injury-related aminoacyl-tRNA synthetase biology, and hepatocellular carcinoma progression via mTORC1 signaling.

Species	Application	Title
human,mouse	WB,IF	Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA Authors - Mirjana Malnar Črnigoj View Article
human,rat	WB	Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Authors - Megha Jhanji View Article
		J Am Chem Soc Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction Authors - Yong Jia Bu View Article
rat	WB	Neural Regen Res A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases. Authors - Byung Sun Park View Article
human,mouse	WB,IHC,IF	Int J Mol Sci FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway Authors - Yaofeng Wang View Article