FARSB Monoclonal antibody

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• FARSB Monoclonal antibody (67924-1-Ig)

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FARSB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67924-1-Ig Copied!

Clone No. 1H10C2

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS

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  WB analysis using 67924-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 67924-1-Ig

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 67924-1-Ig

  WB result of FARSB antibody (67924-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FARSB transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67924-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67924-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67924-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67924-1-Ig (FARSB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 67924-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67924-1-Ig (FARSB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 67924-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U-251 using 67924-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using FARSB antibody (67924-1-Ig, Clone: 1H10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67924-1-Ig targets FARSB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9695

  Product name: Recombinant human FARSB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 240-589 aa of BC017783

  Sequence: DHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINIGPFL

• Full Name: phenylalanyl-tRNA synthetase, beta subunit
• Calculated Molecular Weight: 589 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC017783
• Gene Symbol: FARSB
• Gene ID (NCBI): 10056
• RRID: AB_2918676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NSD9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The FARSB Monoclonal antibody (1H10C2), catalog number 67924-1-Ig, has 1 total citation. The citation appears in Nan Fang Yi Ke Da Xue Xue Bao (2023), where the antibody was used for IHC to study FARSB's role in stratifying prognosis and the cold tumor microenvironment across different cancer types via integrated single-cell and bulk RNA sequencing analysis. The research field is oncology/tumor microenvironment characterization.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]; Authors - Ziran Zhang