FAT4 Polyclonal antibody

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• FAT4 Polyclonal antibody (33468-1-AP)

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  WB analysis using 33468-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 33468-1-AP (FAT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 33468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 33468-1-AP (FAT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 33468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 33468-1-AP (FAT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAT4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33468-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Cadherin family member 14, CDHF14, FAT tumor suppressor homolog 4, FATJ, Fat-like cadherin protein FAT-J

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Tested Applications

• Positive WB detected in: HGC-27 cells, HUVEC cells, MDA-MB-231 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33468-1-AP targets FAT4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37862

  Product name: Recombinant human FAT4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4200-4504 aa of NM_001291285

  Sequence: SVTPDTALSLEGKGRLDYHMSQNEKREYLLRQSLRGAMLEPFGVNSLEVKFRTRSENGVLIHIQESSNYTTVKIKNGKVYFTSDAGIAGKVERNIPEVYVADGHWHTFLIGKNGTATVLSVDRIYNRDIIHPTQDFGGLDVLTISLGGIPPNQAHRDAQTAGFDGCIASMWYGGESLPFSGKHSLASISKTDPSVKIGCRGPNICASNPCWGDLLCINQWYAYRCVPPGDCASHPCQNGGSCEPGLHSGFTCSCPDSHTGRTCEMVVACLGVLCPQGKVCKAGSPAGHVCVLSQGPEEISLPLWA

• Full Name: FAT tumor suppressor homolog 4 (Drosophila)
• Calculated Molecular Weight: 4981aa, 543 kDa
• Observed Molecular Weight: 540 kDa
• GenBank Accession Number: NM_001291285
• Gene Symbol: FAT4
• Gene ID (NCBI): 79633
• RRID: AB_3742983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6V0I7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAT4 is a large transmembrane protein with a mass of approximately 530 kDa. It belongs to the cadherin superfamily and contains multiple cadherin repeats EGF-like domains and one laminin G-like domain. FAT4 is expressed widely across tissues with higher expression observed in the kidney liver and cardiovascular system indicating its involvement in diverse physiological processes. This protein plays a role in maintaining cell adhesion and communication.