FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11394-1-AP

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863

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Validation Data Gallery

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  WB analysis of mouse brain using 11394-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11394-1-AP (FBF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11394-1-AP

  IP result of anti-FBF1 (IP:11394-1-AP, 4ug; Detection:11394-1-AP 1:400) with mouse brain tissue lysate 5000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11394-1-AP targets FBF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBF1 fusion protein Ag1954
• Full Name: Fas (TNFRSF6) binding factor 1
• Calculated Molecular Weight: 1133 aa, 125 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC012332
• Gene Symbol: FBF1
• Gene ID (NCBI): 85302
• RRID: AB_10638320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by the fragment (492-792aa) of FBF1. It recognizes isoform 1, 2, 3, 5 and 6.