Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||mouse lung tissue, human heart tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12188-1-AP targets Fibulin 5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fibulin 5 fusion protein Ag2859|
|Full Name||fibulin 5|
|Calculated molecular weight||448 aa, 50 kDa|
|Observed molecular weight||55 kDa, 65 kDa|
|GenBank accession number||BC022280|
|Gene ID (NCBI)||10516|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.
Arterioscler Thromb Vasc Biol
Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.
Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.
Cell Tissue Res
Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.
Am J Physiol Cell Physiol
Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.
Clin Exp Pharmacol Physiol
Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.
Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.