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  • KD/KO Validated

Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12188-1-AP

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, mouse placenta tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse lung tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12188-1-AP targets Fibulin 5 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibulin 5 fusion protein Ag2859
Full Name fibulin 5
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight 64-66 kDa
GenBank accession numberBC022280
Gene symbol FBLN5
Gene ID (NCBI) 10516
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

Protocols

Product Specific Protocols
WB protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IHC protocol for Fibulin 5 antibody 12188-1-APDownload protocol
IP protocol for Fibulin 5 antibody 12188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Interferon Cytokine Res

Transforming Growth Factor-β-Neutralizing Antibodies Improve Alveolarization in the Oxygen-Exposed Newborn Mouse Lung.

Authors - Sijun Deng
humanWB

Clin Exp Pharmacol Physiol

Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.

Authors - Wei Zhang
  • KD Validated
mouseWB

Am J Physiol Cell Physiol

Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.

Authors - Varadarajan Sudhahar
mouseWB, IHC

Cell Tissue Res

Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.

Authors - Wenli Han
mouseWB

Arterioscler Thromb Vasc Biol

Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.

Authors - Chen Ju-Yi JY