FBP1 Recombinant monoclonal antibody

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• FBP1 Recombinant monoclonal antibody (87898-1-RR)

FBP1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87898-1-RR

Clone No.253535C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, EC:3.1.3.11, FBP, FBPase 1, Fructose-1,6-bisphosphatase 1

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  WB analysis using 87898-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87898-1-RR (FBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: RT-4 cells, HT-29 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87898-1-RR targets FBP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3837

  Product name: Recombinant human FBP1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-338 aa of BC012927

  Sequence: MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

• Full Name: fructose-1,6-bisphosphatase 1
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC012927
• Gene Symbol: FBP1
• Gene ID (NCBI): 2203
• RRID: AB_3745815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09467
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).