FBP2 Polyclonal antibody

FBP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25192-1-AP

Synonyms

FBP2, FBPase 2, fructose 1,6 bisphosphatase 2

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 25192-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25192-1-AP (FBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25192-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25192-1-AP (FBP2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 25192-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25192-1-AP (FBP2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 25192-1-AP is FBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBP2 fusion protein Ag18245
• Full Name: fructose-1,6-bisphosphatase 2
• Calculated molecular weight: 339 aa, 37 kDa
• Observed molecular weight: 37 kDa
• GenBank accession number: BC113632
• Gene symbol: FBP2
• Gene ID (NCBI): 8789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Food Funct; Free fatty acids induce the demethylation of the fructose 1,6-biphosphatase 2 gene promoter and potentiate its expression in hepatocytes.; Authors - Lujing Wang