FBP2 Polyclonal antibody

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  WB analysis using 25192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25192-1-AP (FBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25192-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25192-1-AP (FBP2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 25192-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25192-1-AP (FBP2 antibody) at dilution of 1:200 (under 40x lens).

FBP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25192-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

25192-1-AP targets FBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBP2 fusion protein Ag18245
• Full Name: fructose-1,6-bisphosphatase 2
• Calculated Molecular Weight: 339 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC113632
• Gene Symbol: FBP2
• Gene ID (NCBI): 8789
• RRID: AB_2879951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1 ) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

Publications

Species	Application	Title
human	IF	Food Funct; Free fatty acids induce the demethylation of the fructose 1,6-biphosphatase 2 gene promoter and potentiate its expression in hepatocytes.; Authors - Lujing Wang
mouse	WB	Sci Rep; Identification of mouse soleus muscle proteins altered in response to changes in gravity loading; Authors - Yoko Ino
mouse	WB	FASEB J; Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin-2 causes contractile dysfunction in skeletal muscle; Authors - Syeda N Lamia