fibrosin Polyclonal antibody

fibrosin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11356-1-AP

Synonyms

FBRS, FBS, FBS1, FBSH, fibrosin, Probable fibrosin-1

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Validation Data Gallery

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  WB analysis of Jurkat using 11356-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11356-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11356-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11356-1-AP (fibrosin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11356-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11356-1-AP (fibrosin antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11356-1-AP

  Immunofluorescent analysis of HepG2 cells, using FBRS antibody 11356-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, mouse kidney tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11356-1-AP targets fibrosin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fibrosin fusion protein Ag1870
• Full Name: fibrosin
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC014212
• Gene Symbol: FBRS
• Gene ID (NCBI): 64319
• RRID: AB_10697650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrosin is a lymphokine secreted by activated lymphocytes that induces fibroblast proliferation FBRS (or FBS1) gene, encoding fibrosin (or fibrosin-1), was first cloned by Prakash et al. and was found to be able to stimulate several biological activities related to scarring as a fibrogenic lymphokine. Fibrosin is secreted by activated lymphocytes that induce fibroblast proliferation. In vitro application of fibrosin stimulates expression of alpha smooth muscle actin fibers and plays an important role in up regulating the appearance of myofibroblasts during wound healing and possibly in fibrotic diseases. Fibrosin is also implicated in alcohol-induced hepatic fibrosis in vivo.