FBXL13 Polyclonal antibody

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  IHC staining of human liver using 17139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17139-1-AP (FBXL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17139-1-AP (FBXL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXL13 Polyclonal Antibody for IHC, ELISA

Cat No. 17139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

DRC6, Dynein regulatory complex subunit 6, FBL13, F-box and leucine-rich repeat protein 13, F-box/LRR-repeat protein 13

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17139-1-AP targets FBXL13 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10903

  Product name: Recombinant human FBXL13 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 358-707 aa of BC031285

  Sequence: NDMPTLTDNCVKALVEKCSRITSLVFTGAPHISDCTFRALSACKLRKIRFEGNKRVTDASFKFIDKNYPNLSHIYMADCKGITDSSLRSLSPLKQLTVLNLANCVRIGDMGLKQFLDGPASMRIRELNLSNCVRLSDASVMKLSERCPNLNYLSLRNCEHLTAQGIGYIVNIFSLVSIDLSGTDISNEAFCKSSLILEHLDVSYCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHILDISGCVLLTDQILEDLQIGCKQLRILKMQYCTNISKKAAQRMSSKVQQQEYNTNGPPRWFGYDREGNPVTELDNITSSKGALELTVKKSTYSSEDQAA

• Full Name: F-box and leucine-rich repeat protein 13
• Calculated Molecular Weight: 707 aa, 81 kDa
• GenBank Accession Number: BC031285
• Gene Symbol: FBXL13
• Gene ID (NCBI): 222235
• RRID: AB_2103869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEE6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXL13, a binding determinant of SCF (SKP1-CUL1-F-box)-family E3 ubiquitin ligases, is enriched at centrosomes and interacts with the centrosomal proteins Centrin-2, Centrin-3, CEP152 and CEP192. Induced FBXL13 expression downregulates centrosomal γ-tubulin and disrupts centrosomal microtubule arrays. FBXL13 is a novel regulator of microtubule nucleation activity and participate in promoting cell motility with potential tumour-promoting implications (PMID: 29348145).