FBXL13 Recombinant monoclonal antibody

• 

  WB analysis using 86857-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86857-1-RR (FBXL13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

FBXL13 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86857-1-RR

Clone No.251880F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DRC6, Dynein regulatory complex subunit 6, FBL13, F-box and leucine-rich repeat protein 13, F-box/LRR-repeat protein 13

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, BGC-823 cells, K-562 cells, HuH-7 cells, HGC-27 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86857-1-RR targets FBXL13 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10903

  Product name: Recombinant human FBXL13 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 358-707 aa of BC031285

  Sequence: NDMPTLTDNCVKALVEKCSRITSLVFTGAPHISDCTFRALSACKLRKIRFEGNKRVTDASFKFIDKNYPNLSHIYMADCKGITDSSLRSLSPLKQLTVLNLANCVRIGDMGLKQFLDGPASMRIRELNLSNCVRLSDASVMKLSERCPNLNYLSLRNCEHLTAQGIGYIVNIFSLVSIDLSGTDISNEAFCKSSLILEHLDVSYCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHILDISGCVLLTDQILEDLQIGCKQLRILKMQYCTNISKKAAQRMSSKVQQQEYNTNGPPRWFGYDREGNPVTELDNITSSKGALELTVKKSTYSSEDQAA

• Full Name: F-box and leucine-rich repeat protein 13
• Calculated Molecular Weight: 707 aa, 81 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC031285
• Gene Symbol: FBXL13
• Gene ID (NCBI): 222235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NEE6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXL13, also known as F-box and leucine-rich repeat protein 13, is a human protein-coding gene located on chromosome 7q22.1. It is a member of the F-box protein family, characterized by an approximately 40-amino acid F-box motif. FBXL13 is part of the SCF-type E3 ubiquitin ligase complex, which plays a role in protein ubiquitination and degradation. The gene has multiple isoforms, including 84 kDa, 81 kDa, 79 kDa, and 53 kDa variants. While its specific function within the complex is not fully understood, FBXL13 has been implicated in regulating centrosome homeostasis and cell migration by directing the proteolysis of CEP192. Additionally, it may play a role in circadian rhythms by ubiquitinating and mediating the degradation of cryptochrome proteins.