Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25950-1-AP targets FBXL16 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22827 Product name: Recombinant human FBXL16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-479 aa of BC036680 Sequence: WRRVLYQPKFWAGLTPVLHAKELYNVLPGGEKEFVNLQGFAARGFEGFCLVGVSDLDICEFIDNYALSKKGVKAMSLKRSTITDAGLEVMLEQMQGVVRLELSGCNDFTEAGLWSSLSARITSLSVSDCINVADDAIAAISQLLPNLAELSLQAYHVTDTALAYFTARQGHSTHTLRLLSCWEITNHGVVNVVHSLPNLTALSLSGCSKVTDDGVELVAENLRKLRSLDLSWCPRITDMALEYVACDLHRLEELVLDRCVRITDTGLSYLSTMSSLRSLYLRWCCQVQDFGLKHLLALGSLRLLSPAGCPLLTTTGLSGLVQLQELEELELTNCPGATPELFKYFSQHLPRCLVIE Predict reactive species |
| Full Name | F-box and leucine-rich repeat protein 16 |
| Calculated Molecular Weight | 479 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC036680 |
| Gene Symbol | FBXL16 |
| Gene ID (NCBI) | 146330 |
| RRID | AB_2880307 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N461 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The FBXL16 Polyclonal antibody (catalog number 25950-1-AP) has 1 total citation. It appears in Nature Chemical Biology (2024), a high-impact journal. The associated research field is the Hippo signaling pathway, specifically examining how LATS2 condensates organize signalosomes for Hippo pathway signal transduction, using IHC in human samples.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol LATS2 condensates organize signalosomes for Hippo pathway signal transduction |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The customer used it for Western Blot and reported that the product worked just as expected. They also praised the fast shipping and helpful customer service, noting they would recommend the company to colleagues in their lab. Overall, the feedback reflects satisfaction with both the product performance and the purchasing experience.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nazia (Verified Customer) (01-06-2022) | Package arrived very quickly and staff were very helpful. Product worked just as expected. Great customer service. I will be recommending ProteinTech to colleagues in my lab.
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