FBXO17 Polyclonal antibody

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  WB analysis using 12844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12844-1-AP

  WB result of FBXO17 antibody (12844-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO17 transfected HepG2 cells.

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  WB analysis of mouse heart using 12844-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12844-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (12844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 12844-1-AP

  Immunofluorescent analysis of HeLa cells using 12844-1-AP (FBXO17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

FBXO17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12844-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FBG4, F-box only protein 17, F-box only protein 26, FBX17, FBX26

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Tested Applications

• Positive WB detected in: U-251 cells, mouse heart tissue, HepG2 cells, mouse lung tissue
• Positive IHC detected in: human heart tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

12844-1-AP targets FBXO17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3867

  Product name: Recombinant human FBXO17 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-278 aa of BC012385

  Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS

• Full Name: F-box protein 17
• Calculated Molecular Weight: 278 aa, 31 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC012385
• Gene Symbol: FBXO17
• Gene ID (NCBI): 115290
• RRID: AB_2877889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EF6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
mouse	WB	J Biol Chem; SCFFBXO17 E3 ligase modulates inflammation by regulating proteasomal degradation of glycogen synthase kinase-3β in lung epithelia.; Authors - Tomeka Suber
human	IHC	Carcinogenesis; Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma.; Authors - Rui-Chao Chai
human	WB,IHC	J Gene Med; Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217.; Authors - Siyao Shen
human	WB	Biosci Biotechnol Biochem; F-box protein 17 promotes glioma progression by regulating glycolysis pathway.; Authors - Chao Deng ; KD Validated
mouse,human	WB,IHC	Int J Med Sci; FBXO17 Inhibits the Wnt/β-Catenin Pathway and Proliferation of Ishikawa Cells; Authors - Zi-Meng Zheng