FBXO41 Polyclonal antibody

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• FBXO41 Polyclonal antibody (24519-1-AP)

FBXO41 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 24519-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

F box only protein 41, F box protein 41, Fbx41, FBXO41, KIAA1940

• 

  WB analysis using 24519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24519-1-AP (FBXO41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 24519-1-AP

  IP result of anti-FBXO41 (IP:24519-1-AP, 4ug; Detection:24519-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 24519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24519-1-AP (FBXO41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 24519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24519-1-AP (FBXO41 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24519-1-AP targets FBXO41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21595

  Product name: Recombinant human FBXO41 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 531-875 aa of BC112202

  Sequence: GRRAERVSPSRSNEVISPEILKMRAALFCIFTYLDTRTLLHAAEVCRDWRFVARHPAVWTRVLLENARVCSKFLAMLAQWCTQAHSLTLQNLKPRQRGKKESKEEYARSTRGCLEAGLESLLKAAGGNLLILRISHCPNILTDRSLWLASCYCRALQAVTYRSATDPVGHEVIWALGAGCREIVSLQVAPLHPCQQPTRFSNRCLQMIGRCWPHLRALGVGGAGCGVQGLASLARNCMRLQVLELDHVSEITQEVAAEVCREGLKGLEMLVLTATPVTPKALLHFNSICRNLKSIVVQIGIADYFKEPSSPEAQKLFEDMVTKLQALRRRPGFSKILHIKVEGGC

• Full Name: F-box protein 41
• Calculated Molecular Weight: 875 aa, 94 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC112202
• Gene Symbol: FBXO41
• Gene ID (NCBI): 150726
• RRID: AB_2879586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TF61
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO41 also named as F box only protein 41 is a 875 amino-acid protein, which directly interact with SKP1 and CUL1. FBXO41 is identified as a Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, which as a regulator mediates some protein ubiquitination degradation. FBXO41 consists of F-box domain and has zinc ion binding activity. FBXO41 may have an association with Parkinson's disease.

Publications

What published studies show

The FBXO41 Polyclonal antibody (Cat# 24519-1-AP) has 3 total citations published in Science Advances, iScience, and eLife. The cited research spans neurobiology and Parkinson's disease (mitochondrial ubiquitylation and Parkin/PINK1 pathway), synaptic transmission and hippocampal network maturation, and aging proteomics (chromatin proteome signatures across mouse organs). All citations used the antibody for Western blot applications in human, rat, or mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
rat	WB	iScience; Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation.; Authors - Ana R A A Quadros
Mouse	WB	Elife; Distinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions; Authors - Giorgio Oliviero