FBXO5 Polyclonal antibody

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• FBXO5 Polyclonal antibody (10872-1-AP)

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10872-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, FBX5, FBXO5

• 

  WB analysis of HEK-293 using 10872-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10872-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10872-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 10872-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human ovary using 10872-1-AP

  human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 10872-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 10872-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10872-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, human ovary tissue, K-562 cells, mouse kidney tissue, rat liver tissue
• Positive IHC detected in: human placenta tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10872-1-AP targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1296

  Product name: Recombinant human FBXO5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-447 aa of BC018905

  Sequence: MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVELETESKRLHNKENQHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTKKSKKNLRRL

• Full Name: F-box protein 5
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 56 kDa, 45 kDa
• GenBank Accession Number: BC018905
• Gene Symbol: FBXO5
• Gene ID (NCBI): 26271
• RRID: AB_2247034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKT4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

Publications

What published studies show

FBXO5 Polyclonal antibody (Cat# 10872-1-AP) has 12 citations published in journals including Nature, Nature Communications, Journal of Cellular and Molecular Medicine, Current Issues in Molecular Biology, Experimental Cell Research, Journal of Cancer Research and Clinical Oncology, Molecular Cell Probes, Discovery Oncology, Pathology – Oncology Research, and Translational Cancer Research. Research fields span cell cycle regulation, DNA replication, and multiple cancer types including glioma, lung, gastric, hepatocellular, prostate, and esophageal cancers.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi
	WB	Nat Commun; The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication; Authors - Kyohei Matsuhashi
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	WB	J Cell Mol Med; An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway.; Authors - Mo Wang
human	WB	Curr Issues Mol Biol; Downregulation of Splicing Factor PTBP1 Curtails FBXO5 Expression to Promote Cellular Senescence in Lung Adenocarcinoma; Authors - Haoyu Li ; KD Validated
human	WB	Exp Cell Res; The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.; Authors - Pan Wei