FBXO5 Polyclonal antibody

FBXO5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10872-1-AP

Synonyms

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, FBX5, FBXO5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human ovary tissue, K-562 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10872-1-AP targets FBXO5 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO5 fusion protein Ag1296
Full Name F-box protein 5
Calculated molecular weight 54 kDa
Observed molecular weight 56 kDa, 45 kDa
GenBank accession numberBC018905
Gene symbol FBXO5
Gene ID (NCBI) 26271
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

Protocols

Product Specific Protocols
WB protocol for FBXO5 antibody 10872-1-APDownload protocol
IHC protocol for FBXO5 antibody 10872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway.

Authors - Mo Wang
  • KD Validated
humanWB

Nature

CRL4AMBRA1 is a master regulator of D-type cyclins.

Authors - Daniele Simoneschi